Lab Protocols

Plastic labware is a crucial ingredient of any laboratory. So it is imperative that all the plastic labware is properly cleaned and cared for to get the right result. In this page different aspects of caring as well as cleaning of plasticware has been provided to guide the users to accurate experiment results. Effect of Chemicals on Plastic Labware Certain types of chemicals like oils, detergents, lubricants,pure water and surface additives in presence of tensile stress can result in cracking of the plastic ware. Extended exposure to strong oxidising agents are also known to cause embrittlement and failure. A Chemical affects the strength, flexibility, appearance, dimensions as well as weight of plastic labware. The intensity depends upon. Length of exposure Temperature Concentration  Effect of Heat on Plastic Labware Plastic labware should never be allowed to come in direct contact with a flame or put on a hotplate surface....

For getting successful results from the experiments performed in a laboratory, it is essential that the lab glassware gets perfectly cleaned. However cleaning laboratory glassware is not a very easy task. Laboratory glassware cleaning has to be done physically as well as chemically so that they become free of any germs. However it is to be kept in mind that as such there is no single best laboratory glassware cleaning solution that can be termed as the "best" for all conditions. Laboratory Glassware Cleaning Basics Laboratory Glassware Cleaning If the lab glassware is cleaned immediately after use then the problems associated with dirt can be controlled to a great extent. If detergent is used for laboratory glassware washing, it should be the one that is specially designed for lab glassware cleaning. These detergents are always to be used in place of the normal dish washing detergents used in homes. It has been seen that for cleani...

Fritted glassware used in the labs unless properly taken care of and used in proper conditions can result in breakage as well as inaccurate results. This section highlights some of the important things regarding the cleaning and usage of Fritted Glassware. Cleaning of Frittedware A new fritted ware like a filter is required to be washed by suction. For this hot hydrochloric acid is used and followed by rinsing with water. This treatment effectively removes loose particles like dust. Like all glassware it is also advisable to clean all Fritted ware as soon as possible after use. This helps to extend their life. For a Fritted filter most of the precipitates gets removed from the filter surface with a simple rinsing by water. However this is to be done from the reverse side with a water pressure not exceeding 15 lbs./sq. in. Alternatively, water is drawn through the filter from the reverse side using a vacuum pump is equally effec...

A plastic labware that is used repeatedly for experiments needs to be free from any contaminants to ensure a precise result. To ensure this plastic labware are sterilised. In this section different techniques of sterilisation are discussed. Autoclaving : Autoclaving of plastic labware is done at 121°C, 15 psi for half an hour. Before autoclaving process ensure that the caps are properly loosened or removed so as to check any accidental implosion. It has been seen that few chemicals with no appreciable effect on plastic resins at room temperature causes deterioration during autoclaving temperatures. That is why they must be removed with distilled water prior to autoclaving. Resins like Polymethylpentene, PFA, Polypropylene, polypropylene copolymer can be autoclaved repeatedly at 121°C/15 psi with a 15 minutes cycle length. Polysulfone (PSF) and polycarbonate (PC) although autoclavable, but gets we...

If you want to prolong the life of laboratory utensils made of stainless steel then it is necessary to take proper care of them. The following points given below give some useful hints in caring for stainless steel laboratory utensils. Using Proper Tools : While cleaning stainless steel products , it is better to use non-abrasive tools. Soft cloths and plastic scouring pads if used does not cause any damage to the steel’s passive layer. Using stainless steel pads is also a good option but the scrubbing motion must be directed towards the polishing marks. Cleaning Along the Polish Lines : Often some types of stainless steel lab products come with polishing lines or “grain”which are visible. If visible lines are seen then scrubbing should be done in a motion which is parallel to them. In case grains are not seen a soft cloth or plastic scouring pad can be used. Use of Appropriate Cleaners : For stai...

Safety Signs are of a paramount importance in all labs. It is very essential to povide complete protection to those working in laboratories. The handling of different chamicals, acids and other substances is to be done with great care as these have some inherent features which can be harmful to health. As such all laboratories, whether in schools, universities, hospitals, health centers, test centers should promote health and safety by proper labeling of laboratory equipments, including lab safety signs and symbols. The following are some of the important signs that can be found in the science laboratories warning the users about the possible dangers. Sign for combustible material Sign for corrosive material Sign for toxic chemicals Sign for environmental hazard Sign for eyewash station ...

Laboratory safety equipment is a must in any laboratory especially those dealing in chemical or bio-hazard labs. These days owing to constant research, laboratory safety equipment & lab safety supply are coming with more advanced features that minimizes the risk factors. Whatever be the safety equipment available what is needed is proper validation and regular checks to keep them in operating condition so as they can be easily used when necessary. There are different laboratory safety equipments for varied purpose. Laboratories are places of researches and experiments. Here new things are discovered and invented. In the process the researchers, scientists or lab professional come across a number of equipments, apparatus, samples and chemicals. Regular dealing with all these things can pose serious health problems to those working in labs. Keeping their health in view, a number of laboratory safety measures are taken, which require different types of lab safety equipment and lab ...

It is very essential to follow safety rules in a laboratory, specially in a chemistry laboratory as most of the chemicals and acids used in various experiments are flammable and can be very dangerous if not properly handled. If proper safety rules are maintained, accidents and casualty can be prevented. Here are some of the basic safety rules, which should be followed while working in a chemistry lab, while handling various chemistry lab equipment: Chemistry Laboratory Safety The most important safety rule to consider wearing appropriate clothing as most accidents occur due to improper dress. The lab assistants or the researchers spill acid on themselves or others, which give a burning sensation and people actually set themselves on fire accidentally. Long and loose clothes are strictly prohibited in labs. Long hair should be properly tied. Wear clothes that are made of materials that fire proof. Lab coat and safety goggles are advised to put on during working in ch...

Some of the important considerations in the process of measurement are described here. Accuracy : By Employing a suitable tool a measure of a physical quantity can be known. The reading's accuracy depends on two factors. The device as well as the person who takes the measurement. Therefore accuracy can mean the degree up to which a measured value conforms with a standard or true value. Least Count : A measuring instrument's accuracy depends upon its least count. Least count of the instrument is the least distance which it can measure accurately. Measuring instruments such as vernier callipers, micrometer, screw gauge, etc. show different levels of accuracy in comparison to each other. For example the least count of a screw gauge is 0.01mm while that of a vernier calliper is 0.1mm.  Significant Figures : Significant figures refer to those digits about which we are perfectly sure of plus those digits about which we are not that sure. However, they have a value which is meani...

It is a known fact for anyone working in a laboratory be it the students, the researchers, scientists or lab assistants, it is necessary to follow safety rules and guidelines for their own safety. All laboratories have their lab safety symbols and lab safety equipments like goggles, mask, dress etc. At the same time, it is also very essential that a laboratory is well-maintained and taken care of so as to prevent any kind of dangers involved while working in a lab. As per rule, there should be routine maintenance and safety checks in a laboratory. Infact, it should be the duty of every person working in a laboratory to do a quick safety check after the completion of each experiment and before the starting of a new one. Or a responsible personnel should be assigned the duty to clean the laboratory and perform simple maintenance for lab care and safety . Laboratory Maintenance Presenting below few points which should be considered for laboratory maintenance: Bench: ...