Lab Protocols

Aims of the Fish Ecology protocol are to: ­ determine the species spatial distribution (total abundances and biomass) according to environmental factors to develop habitat modelling, community studies and ecosystem modelling ­ to gather individual measurements for studying sex and populations differences ­ to collect specimens for identification combining molecular and morphology techniques. 1. Ichthyoplankton All the larval stages Specific work needs to be done on Pleuragramma antarcticum for studying their survival conditions. a. Early life stages The larval stages are according to Koubbi et al. (1990). Eggs / Embryo: spawning to hatching Stage 1: yolk­sac larvae, hatching to complete absorption of yolk sac. Stage 2: preflexion larvae: complete yolk sac absorption...

  Atmospheric Deposition Sample Processing Lab prep: 1. Set aside all necessary bottles, vials, tubes, etc. 2. Locate and cache all necessary glassware and filters. 3. Record bottle numbers on the field datasheet, label N tubes and cation bottles if adequate volume is expected. Label sweater tube trays and sweater boxes. Lab cleanup: 1. Rinse, dry and HCl wash a batch of glassware. 2. ...

ADP flameAA cation Method Detection Limit results (SMQ 3/1/01) K: 0.03ppm K trial (10 reps) S.D. 0.003797 (2.821) 0.0107, MDL 5x the MDL @0.05, this is the Practical Quantitation Limit A new standard range of 0.05-0.5 ppm will need to be used Mg: 0.01ppm Mg trial (10 reps) S.D. 0.00135 (2.821) 0.0038, MDL 5x the MDL @0.02, this is the Practical Quantitation Limit The current standard range of 0.02-0.2 ppm will not change Ca: 0.02ppm Ca trial (10 reps) S.D. 0.006596 (2.821) 0.01967, MDL 5x the MDL @ 0.1, this is the Practical Quantitation Limit The current standard range of 0.5-5 pp...