Common Buffers and Common Buffer Preparations

Buffer pKa Neutral form HCl salt Na+ salt
H3PO4 / NaH2PO4 (pKa1) 2.12 98.0 - 120.0
Glycine (pKa1) 2.34 75.07 111.5 -
Citric acid(pKa1) 3.13


Acetic acid 4.75 60.05 - 82.0
Citric acid(pKa2) 4.76 192.1 - 294.1
MES 6.15 195.2 - 217.2
Cacodylic acid 6.27
H2CO3 / NaHCO3 (pKa1) 6.37 62.01 - 84.01
Citric acid(pKa3) 6.40


Bis-Tris 6.50 209.2 245.7 -
ADA 6.60 190.2 - 212.1
Bis-Tris Propane (pKa1) 6.80 282.4 318.9 -
PIPES 6.80 302.4 - 325.3
ACES 6.90 182.2 218.7 -
Imidazole 7.00 68.1 104.5 -
BES 7.15 213.2 - 235.2
MOPS 7.20 209.3 - 231.2
NaH2PO4 / Na2HPO4 (pKa2) 7.21 120.0* - 142.0*
TES 7.50 229.3 - 251.2
HEPES 7.55 238.3 - 260.3
HEPPSO 7.80 268.3 - 290.3
Triethanolamine 7.80 149.2 185.7 -
Tricine 8.10 179.2 215.6 -
Tris 8.10 121.1 157.6 -
Glycine amide 8.20 74.1 110.6 -
Bicine 8.35 163.2 199.7 -
Glycylglycine (pKa2) 8.40 132.1 168.6 -
TAPS 8.40 243.2 - 265.3
Bis-Tris Propane (pKa2) 9.00 ? 355.3 -
Boric acid (H3BO3 / Na2B4O7) 9.24 61.8 - 201.24
CHES 9.50 207.3 - 229.3
Glycine (pKa2) 9.60 75.07 - 96.1
NaHCO3 / Na2CO3 (pKa2) 10.25 84.01 - 105.99
CAPS 10.40 221.3 - 243.3
Piperidine 11.12

-
Na2HPO4 / Na3PO4 (pKa3) 12.67 142.0* - 164.0*
*The anhydrous molecular weight is reported in the table. Actual molecular weight will depend on the degree of hydration.
Common Buffer Preparations
Buffer Stock Soln Components Amount per Liter Soln. Conc. Stock Soln Final Conc.
PBS
(Phosphate Buffered Saline)
adj. pH ~7.3
10X NaCl
KCl
Na2HPO4×7H2O
KH2PO4
80 g
2 g
11.5 g
2 g
1.37 M
27 mM
43 mM
14 mM
137 mM
2.7 mM
4.3 mM
1.4 mM
SSC
adj. pH ~7.0
20X NaCl
Sodium citrate
175 g
88 g
3 M
0.3 M
150 mM
15 mM
STE
(Saline Tris EDTA)
1X Tris base
NaCl
EDTA (acid)
1.2 g
0.6 g
0.29 g
10 mM
10 mM
1 mM
10 mM
10 mM
1 mM
TAE
(Tris acetate EDTA)
pH ~8.5
50X Tris base
Acetic acid (glacial)
EDTA
242 g
57.1 mL
37.2 g
2 M (Tris acetate)
0.1 M
40 mM (Tris acetate)
2 mM
TBE
(Tris borate EDTA)
pH ~8.0
10X Tris base
Boric acid
EDTA
108 g
55 g
40 mL
(0.5 M pH 8)
0.89 M
0.89 M
0.02 M
89 mM
89 mM
2 mM
TE
(Tris EDTA)
pH ~7.5
1X Tris base
H2 EDTA (acid)
1.2 g
0.29 g
10 mM
1 mM
10 mM
1 mM

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