100% Steinberg’s Solution
100% Steinberg’s Solution (AKA 5xMR)
(makes 2 liters)
40 ml NaCl stock solution (17% w/v NaCl)
20 ml KCl stock solution (0.5% w/v KCl)
20 ml CaCl2 stock solution (0.5% w/v CaCl2)
20 ml MgSO4 stock solution (1.025% w/v MgSO4)
1 ml gentamycin stock solution (4% w/v gentamycin)
2.4 g HEPES
1899 ml ddH2O
Adjust pH to 7.4 - 7.6 and store in refrigerator. Usually, you will make 2-4 liters at a time. To make the required stock solutions,
(makes 2 liters)
40 ml NaCl stock solution (17% w/v NaCl)
20 ml KCl stock solution (0.5% w/v KCl)
20 ml CaCl2 stock solution (0.5% w/v CaCl2)
20 ml MgSO4 stock solution (1.025% w/v MgSO4)
1 ml gentamycin stock solution (4% w/v gentamycin)
2.4 g HEPES
1899 ml ddH2O
Adjust pH to 7.4 - 7.6 and store in refrigerator. Usually, you will make 2-4 liters at a time. To make the required stock solutions,
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