Tricaine Solution (zebrafish anesthesia)

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Tricaine Solution:

400 mg tricaine in 97.9 ml E3

0.26 g Tris in 2.1 ml E3

Adjust pH to ~7

Store this solution in a 50 ml conical tube in the freezer

To use as an anesthetic :

4.2 ml tricaine solution

~100 ml E3

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