Serum Free Culture Media (for eyebud cultures)

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Serum Free Culture Media
(makes 100 ml)

1 ml pen/strep antibiotic cocktail (AKA Fungibact)
0.1 g Albumin, bovine serum (fatty acid free, Sigma# A8806)
39 ml ddH2O
60 ml L15

Adjust pH to ~7.4. Sterile Filter prior to using; do not autoclave.

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