Making Multiple Panel Images in Photoshop

1) Open Photoshop on the computer
2) Click File > Open
3) Select the images that you want to overlay and open them in photoshop
4) Make sure there is a saved copy of all the original images
5) Save the images under new names before you begin manipulating them, so that you will always have an unchanged original
6) Once you have opened all of your images, decide how many panels you need for your final image, and pick one to be your base image
7) On your base image, go to Image> Canvas Size
8) A new box will open with the height and width of your current canvas
9) Multiply the height or width by the number of images that need to fit within your canvas (This means if you want a three panel image multiply the width by three and do not change the height. If you want a four panel square image, multiply the width and height by two).
10) Once you change the canvas height and width to the desired size, anchor the base image where you want it in the final image panel (Ex. If you are doing a three-panel image, and your base image is the fluorescent overlay, anchor the base image so that it is the furthest image to the right.)
11) Once you have adjusted the canvas size, go to the next image you are adding to the canvas.
12) Go to Image> Select> All
13) Then, go to File> Copy (or Ctrl C)
14) Once you have a copy of the new image, go back to your base image and paste it into the canvas (File> Paste or Ctrl V)
15) The new image will be in the center of the canvas, and it might cover your anchor image
16) Grab the image with the mouse and move it to the desired area on the canvas
17) Repeat steps 11-16 for any other images you want to add to the canvas
18) Now save the overlay image as a photoshop and a JPEG file (make sure that you are saving it under a new name and not replacing your original image).

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