Low [Ca++] Reaggregation Solution with 0.05% Anesthesia for eyebud culture

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Low [Ca++] Reaggregation Solution with 0.05% Anesthesia
(makes 1 liter)

6.81 g NaCl
0.05 g KCl
1.47 g CaCl2 (dihydrate)
1.91 g HEPES
1000 ml ddH2O
0.5 g Ethyl 3-aminobenzoate methanesulfonic acid salt (C9H11NO2*CH4SO3)
Phenol Red (a few drops to indicate pH)

Adjust pH to ~7.4. Sterile Filter prior to using; do not autoclave.

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