Cysteine Solution (to remove jelly coat from tad eggs)

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This solution is used to remove the jelly coat from fertilized Xenopus laevis eggs, allowing you to separate out the good eggs.

Cysteine Solution
(makes enough to cysteine 1 petri dish of fertilized eggs)

0.6 g Cysteine
1.0 g Tris base
30 ml 20% Steinberg’s solution

For 4 dishes, and with our current (2009) basic-leaning water:

1.8g Cysteine
1.7g Tris base
90 ml 20% Steinberg's solution

***Resulting pH: ~7.52

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