Culture Media (for eyebud cultures)

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Culture Media
(makes 100 ml)

10 ml Fetal Bovine Serum (FBS)
1 ml pen/strep antibiotic cocktail (AKA Fungibact)
1 ml Embryo Extract solution (EE)
28 ml ddH2O
60 ml L15

Adjust pH to ~7.4. Sterile Filter prior to using; do not autoclave.

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