AChE stain solution

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AChE stain solution
(makes ~98 ml)

AChE Solution 1 (will be blue)
40 ml 0.1 M acetate buffer
28 mL ddH2O
3.2 mL glycine/copper solution
2 ml 8.8 mM promethazine

AChE Solution 2 (will be brown/yellow)
18 ml ddH2O
372 mg acetylthiocoline iodide
6 ml 0.1 M copper sulfate

Both solutions must be made fresh each time. Filter solution 2 with filter paper and then mix with solution 1. Cover tissue/slides in the combined solution and place on rocker/belly dancer for 24-72 hours, then rinse tissue/slides in ddH2O for five minutes. Repeat rinse three times.

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