Acetate Buffer

This solution is used in AChE stain solution.

Acetate Buffer
(makes 200 ml)

1.353 g NaC2H3O2 (or 0.82 g / 100 ml ddH2O)
0.1995 ml glacial acetic acid (or 0.57 ml / 100 ml ddH2O)
200 ml ddH2O (or 165 ml of sodium solution and 35 ml of acid solution)

Adjust pH to 5.3.