1X Modified Barth’s Saline (MBS)

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100 ml 10X MBS stock
1.25 ml 4% gentamycin (1 g gentamycin sulfate in 25 ml ddH2O)
893 ml ddH2O
7 ml autoclaved 0.1M CaCl2 (11.1 g CaCl2 / 100 ml ddH2O)

Adjust pH to ~7.4. Sterile filter prior to use. Make fresh weekly and store in refrigerator.

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