10X Tadpole Anesthesia (0.5%)

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use this anesthesia to anesthetize adult frogs before dissecting out testes, and also for 1X dilution.


10X Tadpole Anesthesia (0.5%) (AKA Adult Anesthetic, MS222, Tricane)


0.5 g Ethyl 3-aminobenzoate methanesulfonic acid salt (C9H11NO2*CH4SO3)
100 ml 20% Steinberg’s solution

Initially the solution is very acidic. Be careful adjusting the pH, as it tends to jump quickly past pH 4.5. Our meter has trouble reading anesthesia and will never truly stabilize. Once the “S” symbol appears on meter, use pH paper to adjust pH to ~7.4.

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