NASCO injection schedule FOR XENOPUS BREEDING

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Make up HCG solution of 500IU per 1 mL.
(in 2500IU vial add 5cc 0.9% saline)

Store HCG solution at 4C for up to three months.


Day 1:
Morning (9am)  – Inject male with 150IU. Place in separate tank.
Day 2:
              Morning (9am)  – Inject female with 250IU.  Place in breeding tank.
                                       Inject male with 150IU.  Return to separate tank.
              Evening (5pm) – Inject female with 500IU.  Return to breeding tank.
                                         Place male into breeding tank with female.
Day 3:  Collect lots of fertilized eggs.  If no eggs, re-inject female with 500IU  and re-inject male with 150IU
 

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