DAPI Staining protocol
Prepare the following solutions:
(To determine the amount needed for your experiment, please note that one tube of Solution 1 will yield a total of 100mL of working solution, at a final concentration of 0.5ug/mL.)
Solution 1*:
5 μL DAPI (10 mg/ml solution)
995 μL PB
* Solution is viable for one month if stored at 4° in the dark
Solution 2 (working solution):
10 μL of Solution 1
990 μL PB
Treatment: →Add Solution 2 (working solution) to your tissue/cells and incubate at room temperature for
1- 5 minutes.
→Rinse 2X with PB.
→Mount coverslip with Shur/Mount, Prolong or comparable antifade medium.
→Seal coverslips (if necessary) with Valap or nail polish.**
**Try to avoid nail polish if are visualizing GFP, as the additives in nail polish tend to quench the signal.
(To determine the amount needed for your experiment, please note that one tube of Solution 1 will yield a total of 100mL of working solution, at a final concentration of 0.5ug/mL.)
Solution 1*:
5 μL DAPI (10 mg/ml solution)
995 μL PB
* Solution is viable for one month if stored at 4° in the dark
Solution 2 (working solution):
10 μL of Solution 1
990 μL PB
Treatment: →Add Solution 2 (working solution) to your tissue/cells and incubate at room temperature for
1- 5 minutes.
→Rinse 2X with PB.
→Mount coverslip with Shur/Mount, Prolong or comparable antifade medium.
→Seal coverslips (if necessary) with Valap or nail polish.**
**Try to avoid nail polish if are visualizing GFP, as the additives in nail polish tend to quench the signal.
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