basic Immunohistochemistry protocol

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1. Incubate 2 x 20 min. in 0.1M PB at R.T.

2. Incubate 4 x 10 min in 0.1M PB + 0.3% triton at R.T.

3. Incubate 30 min in 0.1M PB + 0.3% triton +5% NGS at RT

4. Incubate overnight with primary antibody _______________

Diluted _____________ in 0.1M PB + 0.03% triton at 40C.

5. Rinse 4 x 10 min in 0.1M PB + 0.3% triton at RT

6. Incubate slides 1 hr in secondary antibody _______________

Diluted _____________ in 0.1M PB + 0.03% triton at R.T.

7. Rinse 3 x. 10 min in 0.1M PB at R.T.

8. 3 drops mounting medium + coverslip. Store at –200C

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