0.3% PTU (to prevent tad pigmentation)
Treat tads with ~1.5 ml PTU prior to stage 30-32 to prevent pigmentation.
0.3% PTU
0.3 g Phenylthiocarbamide (Sigma # P-7629, also called Phenylthiourea)
100 ml 20% Steinberg’s solution
Some of the PTU will not dissolve. Shake solution prior to use to dissolve some of the precipitate (put “shake well” on label). Store in refrigerator.
0.3% PTU
0.3 g Phenylthiocarbamide (Sigma # P-7629, also called Phenylthiourea)
100 ml 20% Steinberg’s solution
Some of the PTU will not dissolve. Shake solution prior to use to dissolve some of the precipitate (put “shake well” on label). Store in refrigerator.
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