Molecular weight standards used for SDS-PAGE:

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The molecular weight  standards used in the  SDS-polyacrylamide gel electrophoresis and Western blotting were, "Color Markers, Wide Range" (Sigma).
  The dye attachment increases the apparent molecular weights of the modified proteins.  The column headed "Apparent Molecular Weight (in Daltons)" is the estimate provided by Sigma for their apparent molecular weights when used as standards in SDS-PAGE.  My personal experience is that the apparent molecular weights actually determined by SDS-PAGE are usually somewhere between the actual protein molecular weights and the estimates from Sigma.  Life is never simple, is it?
 
Protein Molecular Weight 
(in Daltons) 		    Color  Apparent Molecular Weight
(in Daltons)
Myosin, Rabbit Muscle 205,000 Blue 225,000
beta-Galactosidase (E.coli) 116,000 Turquoise 128,000
Albumin, Bovine Serum 66,000 Pink 73,000
Ovalbumin, chicken egg 45,000 Yellow 50,000
Carbonic Anhydrase, bovine 29,000 Orange 31,000
Trypsin Inhibitor, soybean 20,000 Green 26,000
Lactalbumin, bovine milk 14,200 Purple 20,000
Aprotinin, bovine milk 6,500 Blue 10,000

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