Aiba and Ogawa (AO) Medium - Cyanobacteria Media Recipes

-
This media is prepared as two separate solutions to avoid precipitation: Solution A:
NaCl 1.0 g
MgSO4 7H2O 0.2 g
K2SO4 1.0 g
CaCl2 2H2O 40 mg
NaNO3 2.5 g
FeSO4 7H2O 10 mg
Gaffron micronutrients 1.0 ml
Deionized water to 500 ml
Solution B:
K2HPO4 0.5 g
Na2CO3 4.03 g
NaHCO3 13.61 g
Deionized water to 500 ml
After autoclaving, mix Solution A and Solution B asceptically. Final pH 9.4-9.8.
Gaffron micronutrients:
H3BO3 3.1 g
MnSO4 4H2O 2.23 g
ZnSO4 7H2O 0.22 g
(NH4)6Mo7O24 4H2O 0.088 g
Co(NO3)2 6H2O 0.146 g
VOSO4 6H2O 0.054 g
Al2(SO4)3K2SO4 2H2O 0.474 g
NiSO4(NH4)2SO4 6H2O 0.198 g
Cd(NO3)2 4H2O 0.154 g
Cr(NO3)3 7H2O 0.037 g
Na2WO4 2H2O 0.033 g
KBr 0.119 g
KI 0.083 g
Deionized water to 1 L

Comments

Post a Comment

Popular posts from this blog

Prepare 6X Laemmli buffer

-
Laemmli is a sample buffer to use in western blot. 6X sample buffer is added to each protein sample and is boiled or heated for 5-10 minutes. Recipe to prepare 10 ml: - 1.2gr SDS (sodium dodecyl sulfate) - 6mg bromophenol blue - 4.7ml glycerol - 1.2ml Tris 0.5M pH6.8 - 2.1ml dwater warm it a little bit and shake it till everything is dissolved. It takes time. - add 0.93gr DTT. Wait until it is completely dissolved. Aliquote and keep frozen at-20ºC.
Read more

0.1 M Triethanolamine

-
0.1 M TEA: TEA = Triethanolamine You will dilute TEA 1:10 in Millipore water: Pour 50 mL TEA into a 50 mL conical tube (TEA is very viscuous, so best to measure it in something that is disposable). Pour TEA from conical tube into 450 mL Millipore water with stirring. pH to 8.0
Read more

Laboratory Maintenance Checklist

-
Image
It is a known fact for anyone working in a laboratory be it the students, the researchers, scientists or lab assistants, it is necessary to follow safety rules and guidelines for their own safety. All laboratories have their lab safety symbols and lab safety equipments like goggles, mask, dress etc. At the same time, it is also very essential that a laboratory is well-maintained and taken care of so as to prevent any kind of dangers involved while working in a lab. As per rule, there should be routine maintenance and safety checks in a laboratory. Infact, it should be the duty of every person working in a laboratory to do a quick safety check after the completion of each experiment and before the starting of a new one. Or a responsible personnel should be assigned the duty to clean the laboratory and perform simple maintenance for lab care and safety . Laboratory Maintenance Presenting below few points which should be considered for laboratory maintenance: Bench:
Read more