Method for spotting yeast cells for cell growth assay

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Hahn Lab - Methods - Method for spotting yeast cells for cell growth assay
Method for spotting yeast cells for cell growth assay
Ted Young, 2009

Fill wells of 96 well microplate with 240 microliters 10% glycerol.

Add 60 microliters culture. Make five fold serial dilutions by removing 60 microliters of first dilution and pipeting into 240 microliters and so on.

Insert yeast multi-pin apparatus into 96 well plate. Gently pull out being careful not to touch sides of the plate. Quickly put on Petri plate.

This will give uniform plating of cells.

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