Prepare 6X Laemmli buffer

Written By India Gate on Wednesday, May 25, 2011 | 4:45 PM

Laemmli is a sample buffer to use in western blot. 6X sample buffer is added to each protein sample and is boiled or heated for 5-10 minutes.

Recipe to prepare 10 ml:

- 1.2gr SDS (sodium dodecyl sulfate)
- 6mg bromophenol blue
- 4.7ml glycerol
- 1.2ml Tris 0.5M pH6.8
- 2.1ml dwater
warm it a little bit and shake it till everything is dissolved. It takes time.
- add 0.93gr DTT.
Wait until it is completely dissolved.

Aliquote and keep frozen at-20ÂșC.