How to Overlay Fluorescent Images in Photoshop

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1) Open Photoshop on the computer
2) Click File > Open
3) Select the images that you want to overlay and open them in photoshop
4) Make sure there is a saved copy of all the original images
5) Save the images under new names before you begin manipulating them, so that you will always have an unchanged original
6) Once you have opened all of your images, pick one to be your base image (In this example, the base image will be green, and the other two images will be red and blue).
7) Bring the blue fluoresent image to the front
8) Go to Image> Select> All
9) Then, go to File> Copy (or Ctrl C)
10) Once you have a copy of the blue image, go back to your base green image and click on the channels tab (next to the layers tab at the bottom right)
11) Highlight the blue channel
12) Paste (File> Paste or Ctrl V) your copied blue image into the blue channel
13) Repeat these steps copying the red image into the red channel
14) Now save the overlay image as a photoshop and a JPEG file (make sure that you are saving it under a new name and not replacing your original green image).

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