Common Buffers and Common Buffer Preparations
Buffer | pKa | Neutral form | HCl salt | Na+ salt |
H3PO4 / NaH2PO4 (pKa1) | 2.12 | 98.0 | - | 120.0 |
Glycine (pKa1) | 2.34 | 75.07 | 111.5 | - |
Citric acid(pKa1) | 3.13 | |||
Acetic acid | 4.75 | 60.05 | - | 82.0 |
Citric acid(pKa2) | 4.76 | 192.1 | - | 294.1 |
MES | 6.15 | 195.2 | - | 217.2 |
Cacodylic acid | 6.27 | |||
H2CO3 / NaHCO3 (pKa1) | 6.37 | 62.01 | - | 84.01 |
Citric acid(pKa3) | 6.40 | |||
Bis-Tris | 6.50 | 209.2 | 245.7 | - |
ADA | 6.60 | 190.2 | - | 212.1 |
Bis-Tris Propane (pKa1) | 6.80 | 282.4 | 318.9 | - |
PIPES | 6.80 | 302.4 | - | 325.3 |
ACES | 6.90 | 182.2 | 218.7 | - |
Imidazole | 7.00 | 68.1 | 104.5 | - |
BES | 7.15 | 213.2 | - | 235.2 |
MOPS | 7.20 | 209.3 | - | 231.2 |
NaH2PO4 / Na2HPO4 (pKa2) | 7.21 | 120.0* | - | 142.0* |
TES | 7.50 | 229.3 | - | 251.2 |
HEPES | 7.55 | 238.3 | - | 260.3 |
HEPPSO | 7.80 | 268.3 | - | 290.3 |
Triethanolamine | 7.80 | 149.2 | 185.7 | - |
Tricine | 8.10 | 179.2 | 215.6 | - |
Tris | 8.10 | 121.1 | 157.6 | - |
Glycine amide | 8.20 | 74.1 | 110.6 | - |
Bicine | 8.35 | 163.2 | 199.7 | - |
Glycylglycine (pKa2) | 8.40 | 132.1 | 168.6 | - |
TAPS | 8.40 | 243.2 | - | 265.3 |
Bis-Tris Propane (pKa2) | 9.00 | ? | 355.3 | - |
Boric acid (H3BO3 / Na2B4O7) | 9.24 | 61.8 | - | 201.24 |
CHES | 9.50 | 207.3 | - | 229.3 |
Glycine (pKa2) | 9.60 | 75.07 | - | 96.1 |
NaHCO3 / Na2CO3 (pKa2) | 10.25 | 84.01 | - | 105.99 |
CAPS | 10.40 | 221.3 | - | 243.3 |
Piperidine | 11.12 | - | ||
Na2HPO4 / Na3PO4 (pKa3) | 12.67 | 142.0* | - | 164.0* |
Common Buffer Preparations | |||||
Buffer | Stock Soln | Components | Amount per Liter Soln. | Conc. Stock Soln | Final Conc. |
PBS (Phosphate Buffered Saline) adj. pH ~7.3 | 10X | NaCl KCl Na2HPO4×7H2O KH2PO4 | 80 g 2 g 11.5 g 2 g | 1.37 M 27 mM 43 mM 14 mM | 137 mM 2.7 mM 4.3 mM 1.4 mM |
SSC adj. pH ~7.0 | 20X | NaCl Sodium citrate | 175 g 88 g | 3 M 0.3 M | 150 mM 15 mM |
STE (Saline Tris EDTA) | 1X | Tris base NaCl EDTA (acid) | 1.2 g 0.6 g 0.29 g | 10 mM 10 mM 1 mM | 10 mM 10 mM 1 mM |
TAE (Tris acetate EDTA) pH ~8.5 | 50X | Tris base Acetic acid (glacial) EDTA | 242 g 57.1 mL 37.2 g | 2 M (Tris acetate) 0.1 M | 40 mM (Tris acetate) 2 mM |
TBE (Tris borate EDTA) pH ~8.0 | 10X | Tris base Boric acid EDTA | 108 g 55 g 40 mL (0.5 M pH 8) | 0.89 M 0.89 M 0.02 M | 89 mM 89 mM 2 mM |
TE (Tris EDTA) pH ~7.5 | 1X | Tris base H2 EDTA (acid) | 1.2 g 0.29 g | 10 mM 1 mM | 10 mM 1 mM |
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