Internal & External Saline

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Internal Saline
Weight per 10 ml
(mg)
Mw
Final Conc.
(mM)
Cs Meth Sul
182.40
228.0
90
MgCl2
10.17
203.3
5
TEA-Cl
33.14
165.7
20
EGTA
38.04
380.4
10
HEPES
47.66
238.3
20
ATP
11.00
551.1
2
GTP
1.57
          523.2
  0.3

pH= 7.2  (7.15-7.25)                  (use CsOH)
Osmolarity 250                           (use H2O)

  • start with 8ml then fill up to 10 ml
  • Aliquot 500 ml/ tube, then store at –80C for up to 1 month.




External Saline
Weight per 1 L*
(mg)
Mw
Final Conc.
NaCl
6720.6
58.44
115 mM
KCl
298.2
74.55
4 mM
CaCl2-2H2O
441
147.00
3 mM
MgCl2-6H2O
610
203.30
3 mM
HEPES
1191.5
238.30
5 mM
Glycine
.76
75.10
10 mM
Glucose
1802
180.20
10 mM
Picrotoxin**
Add later

100 mM

pH= 7. 2  (7.15-7.25)             (use NaOH,)                                                                                                           
Osmolarity 250-255               (use H2O)

*   start with 800ml then fill up to 1L
** picrotoxin:  240.8 mg in 4 ml water, aliquot in 100 ml, store at –20C

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