Expand (Roche/Boehringer-Mannheim) Long Template PCR

Additional instructions for
Expand (Roche/Boehringer-Mannheim) Long Template PCR


Master Mix I Master Mix II
template enzyme
primers buffer
dNTPs ddH2O to 25 l
ddH2O to 25 l

Notes:
  • Preheat PCR machine to 94°C using instant key
  • Mix the above two for 1 reaction F.V. 50 l
  • Use thin wall 0.2 ml PCR tubes
  • Vortex to mix thoroughly
  • Spin pulse
  • Overlay with 30 l oil even with hot bonnet
  • Master mix I can be mixed in thin wall tube when few samples
  • Master mixes of I or II can be done for large # of reactions

Comments

Popular posts from this blog

Prepare 6X Laemmli buffer

0.1 M Triethanolamine

Laboratory Maintenance Checklist