Expand (Roche/Boehringer-Mannheim) Long Template PCR
Additional instructions for
Expand (Roche/Boehringer-Mannheim) Long Template PCR
Notes:
Expand (Roche/Boehringer-Mannheim) Long Template PCR
Master Mix I | Master Mix II |
template | enzyme |
primers | buffer |
dNTPs | ddH2O to 25 l |
ddH2O to 25 l |
Notes:
- Preheat PCR machine to 94°C using instant key
- Mix the above two for 1 reaction F.V. 50 l
- Use thin wall 0.2 ml PCR tubes
- Vortex to mix thoroughly
- Spin pulse
- Overlay with 30 l oil even with hot bonnet
- Master mix I can be mixed in thin wall tube when few samples
- Master mixes of I or II can be done for large # of reactions
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