Prepare 100ml of Paraformaldehide 4% stock solution
To prepare 100ml of PFA 4% stock solution:
- Add 86ml ddwater in an enlermeyer
- Boil water in the microwave
- Weigh 4g of PFA
From now on in the fume hood
- Transfer PFA in the erlenmeyer
- Add a clean stirring bar
- Add a drop of NaOH 10M
- Stir it and keep it hot (heat at #1-2) till it goes clear (DO NOT LET PFA BOIL)
- Place on ice to cool it down
- Adjust volume to 90ml
- Add 10ml PBS 10X
- Adjust pH to 7.4
Store at RT or 4C
- Add 86ml ddwater in an enlermeyer
- Boil water in the microwave
- Weigh 4g of PFA
From now on in the fume hood
- Transfer PFA in the erlenmeyer
- Add a clean stirring bar
- Add a drop of NaOH 10M
- Stir it and keep it hot (heat at #1-2) till it goes clear (DO NOT LET PFA BOIL)
- Place on ice to cool it down
- Adjust volume to 90ml
- Add 10ml PBS 10X
- Adjust pH to 7.4
Store at RT or 4C
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