Culture Media (for eyebud cultures)
Culture Media
(makes 100 ml)
10 ml Fetal Bovine Serum (FBS)
1 ml pen/strep antibiotic cocktail (AKA Fungibact)
1 ml Embryo Extract solution (EE)
28 ml ddH2O
60 ml L15
Adjust pH to ~7.4. Sterile Filter prior to using; do not autoclave.
(makes 100 ml)
10 ml Fetal Bovine Serum (FBS)
1 ml pen/strep antibiotic cocktail (AKA Fungibact)
1 ml Embryo Extract solution (EE)
28 ml ddH2O
60 ml L15
Adjust pH to ~7.4. Sterile Filter prior to using; do not autoclave.
Comments
Post a Comment