0.3% PTU (to prevent tad pigmentation)

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Treat tads with ~1.5 ml PTU prior to stage 30-32 to prevent pigmentation.

0.3% PTU

0.3 g Phenylthiocarbamide (Sigma # P-7629, also called Phenylthiourea)
100 ml 20% Steinberg’s solution

Some of the PTU will not dissolve. Shake solution prior to use to dissolve some of the precipitate (put “shake well” on label). Store in refrigerator.

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